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FT-NMR SPECTROSCOPY FACILITY AT SAIF
FT-NMR spectroscopy is used to determine the molecular structure based on the chemical environment of the magnetic nuclei like 1H, 13C, 31P, 23Na, 27Al, 43Ca, 37Cl, 79Br, 19F etc., even at low concentrations. This is one of the most powerful nondestructive techniques in elucidating the molecular structure of biological and chemical compounds.
Description:
In FT NMR spectroscopy, a strong RF pulse excites the entire range of precessional frequencies of a given nuclear species whose time response is known as free induction decay (FID) containing all the information. A Fourier transform of FID gives the NMR spectrum. This is a much faster technique compared to CW and hence it is possible to detect weak lines by signal averaging methods. The spectra of samples containing low abundant nuclei like 13C, 31P, etc., are thus easily obtained. Also dynamic studies are possible by relaxation measurements. Various 2D measurements are possible.
Sample
required :
5 mg for 1H and 15 mg for 13C and other nuclei. Solubility: 10 mg/ml for 1H and 50 mg/ml for 13C and others. Solvent must be specified and solubility must be checked before sending the samples in order to save the expensive deuterated solvents. Sample must be free of paramagnetic impurities.
Solvents available: CDCl3, D2O, C6D6,
CD3COCD3 and DMSOd6.
VT Measurements are possible: The limitations are solubility, freezing point, boiling point and availability of the solvent in the deuterated form. For more information on the boiling and freezing points of some of the commonly used solvents click here. Applications :
Widely used in organic chemistry, biology, medicine, pharmaceuticals, etc. for characterization of compounds.
For a small write-up on some basic concepts on NMR spectroscopy click here.
On-line requisition, Requisition form, measurement charges & solvent
charges.
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