The UV-VIS spectrum is due to the electronic transitions of the molecule. This is characteristic of a compound. Qualitative and quantitative estimations of compounds are possible by this non destructive technique.

The Absorbance (A) of a solution at a particular wavelength is given by Beer-Lambert's law ect = A where c is the concentration of the compound , t is the thickness of the cell and e is the molar extinction coefficient characteristic of the compound at a given wavelength. This principle is used for quantitative measurements.

The spectrometer is well suited for samples in the dissolved (solution) form. The spectral range with appropriate solvent is 200-2500 nm and also it can be used to study single crystals and powder samples. This is a double beam instrument controlled by a microprocessor. Base line correction, repetitive scan, kinetics, derivative and concentration modes are possible. Diffuse reflectance is available for solid samples. VT accessory in the range 5-90 degrees Centigrade is available.

 

 

 

 

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